qPCR and ddPCR Assay Services for Accurate Gene Expression Analysis
BioAgilytix offers Real-Time PCR (RT-PCR), Quantitative PCR (qPCR), and Droplet Digital™ PCR (ddPCR™) platforms as part of our extensive technology suite supporting molecular assays. Our molecular laboratories are equipped with the latest generation of PCR equipment including Applied Biosystems QuantStudio 7 Flex or Pro with both 96- and 384-well fast-cycle options and TaqMan Array Cards for qPCR as well as QX200 and QX ONE ddPCR Systems from Bio-Rad.
BioAgilytix’s PCR expertise
Our team of scientific experts collaborate closely with our clients to develop unique molecular solutions for various therapeutic programs. We are well versed in the development, optimization, and validation of complex molecular assays using qPCR and ddPCR to deliver premier molecular services to support sponsors’ testing needs.
Our laboratory spaces are strategically designed to better accommodate PCR workflows with separate facilities for automated DNA and RNA extraction. Our scientists have experience designing assays for a variety of sample types and are experts in biodistribution and persistence assays as well as viral shedding assays in support of ATMPs.
PCR applications: For detecting single or multiple nucleic targets
BioAgilytix’s scientists utilize qPCR and ddPCR assays in our work with biomarkers, pharmacokinetics/pharmacodynamics (PK/PD), and for the bioanalysis of Advanced Therapy Medicinal Products (ATMPs) programs. PCR is an essential method for detecting nucleic acids and has a wide range of clinical and research applications. Our state-of-the-art qPCR and ddPCR systems allow us to customize molecular solutions for our clients using robust methodologies in a streamlined, efficient manner.
How the qPCR platforms work
qPCR tracks the amplification of DNA or RNA (cDNA) in real time through continual measurements of fluorescent molecules (chromophores) activated during the PCR process. For qPCR assays, we leverage the Applied Biosystems QuantStudio technology, which provides enhanced, high-resolution fluorescence detection for precise quantification with multiplexing capabilities. Our molecular suite is equipped with the latest generation of qPCR equipment, including Applied Biosystems QuantStudio 7 Flex or Pro with both 96- and 384-well fast-cycle options as well as TaqMan Array Cards. Our modern qPCR instrumentation allows for the simultaneous measurement of multiple DNA amplification reactions in a single reaction vessel, making it possible to process large batches of samples and multiplex endpoints to increase throughput. This enables reaction volumes at nanoliter levels, creating substantial savings in the amount of reagents and samples needed to perform testing.
How the ddPCR platforms work
The QX200 and QX ONE ddPCR Systems from Bio-Rad are part of BioAgilytix’s extensive portfolio of PCR technologies and automation systems supporting our core genomic services. Our scientists typically turn to this modality to deliver precise and multiplexed PCR analysis for absolute quantification. As a world-class provider of bioanalysis, we often deal with complex assay demands, and the QX ONE ddPCR system platform enables us to effectively address our customers’ multiplex molecular challenges.
The QX ONE ddPCR System can deliver highly sensitive, and reproducible results. The system is fully automated and enables walk-away automation and ease of multiplexing. ddPCR allows for simultaneous target detection without the need for reference materials due to the precision derived from droplet generation methodology. Automation also eliminates pipetting error and leads to further reproducibility.
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